Maximizing Plasmid Stability and Production of Released Proteins in Yersinia enterocolitica
نویسندگان
چکیده
منابع مشابه
Maximizing plasmid stability and production of released proteins in Yersinia enterocolitica.
Virulent serotypes of Yersinia enterocolitica carry a plasmid (pYV) encoding a family of proteins that are released into the medium and whose expression is temperature and calcium regulated. The plasmid is easily lost from cells during their growth in the laboratory. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a monoclonal antibody (3.2C) tha...
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The IgM, IgG, and IgA antibody responses of patients with Yersinia enterocolitica O:3 infection were studied by immunoblotting with plasmid encoded released proteins of Y enterocolitica as the antigens. The results indicate that antibodies of all three classes are most consistently directed against the proteins of molecular weights 25,000 and 36,000. Less than two months after the onset of infe...
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Virulent strains of Yersinia enterocolitica and their plasmidless, avirulent derivatives were examined for their cell surface properties. Increased surface charge and hydrophobicity of Y. enterocolitica were found to be associated with the possession of a 40- to 48-megadalton plasmid. These surface properties were expressed, as were other plasmid-associated properties, at 37 but not at 22 degre...
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Forty-three strains of Yersinia enterocolitica isolated from children with gastroenteritis and 18 laboratory strains were examined for enterotoxin production by using the infant mouse, Y1 adrenal cell, and rabbit ileal loop assay systems. All clinical isolates and seven laboratory strains were found enterotoxigenic in the infant mouse model, but none of the strains produced toxin in the Y1 adre...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.5.1812-1815.1998